Cytometry Specialist I/II

Requisition # 2022-15279
Date Posted 4 months ago(7/26/2022 5:22 PM)
Department
Molecular Biology
Category
Research and Laboratory
Job Type
Full-Time
Name of Lab
Flow

Overview

A full-time Cytometry Specialist I/II position is available in the Flow Cytometry Resource Facility (FCRF) laboratory in the Department of Molecular Biology at Princeton University. The FCRF provides support for Princeton University faculty and student research utilizing state-of-the-art flow cytometry and fluorescence-activated cell sorting (FACS) techniques and instrumentation.  We currently support over 35 Principal Investigator labs on campus working with a wide variety of model systems and assays.  This is an excellent opportunity to join our shared resource lab and to develop/expand career skills in a growing field.  Position level and salary commensurate with experience.

 

This position reports directly to the Flow Cytometry Resource Facility Director.  

Responsibilities

 

  • Assist with and carry out routine single color and advanced multi-color flow cytometry experiments with users (students, research staff, post-docs and faculty) on analytical flow cytometry instruments. 
  • Orient new users to the lab and directly contribute to the successful daily operation of the facility with minimal direction. 
  • Upon demonstration of appropriate knowledge base, training to operate several cell sorters will be provided with the goal of independent execution of a wide variety of FACS sorting experiments in a BSL2+ level facility. 
  • Provide instrument and software training to new and established clients with minimal supervision.  The lab is equipped with Cell Sorters:  BD FACSAria Fusion, Sony Biotechnology MA900, BioRad S3e, UnionBiometrica BioSort; Flow Cytometers: BD FACSymphony A3, BD LSRII; Others:  Z2 Particle Counter, CytoSpin Centrifuge and FACSDiVa, FlowJo, & FCS Express software platforms. 
  • Provide technical assistance and consultation to clients on experimental design, including appropriate sample preparation techniques and necessary control samples. 
  • Perform daily quality control checks on the benchtop flow cytometers and cell sorters. Keep accurate, legible records to monitor instrument performance. In concert with Facility Director, complete scheduled and unscheduled system maintenance as needed. 
  • Assist in maintaining instrument/experiment calendars and become expert in the use of iLab scheduling and usage reporting software. 
  • Have the ability to multi-task in a fast-paced multi-user research environment while maintaining excellent attention to detail. 
  • Troubleshoot instrument and assay performance, assisting researchers with optimizing their experiments and data analysis. 
  • Archive all facility data and maintain workstation computers monthly. 
  • Organize and maintain a resource reference library for users. 
  • Perform routine laboratory duties including ordering of lab supplies with comparison cost sourcing, general lab upkeep, preparation of reagents, disposal of laboratory waste, and routine maintenance of laboratory equipment, including weekly sterilization and monthly cleaning protocols. Must be able to lift 50 pounds. 
  • Other duties as assigned by the FCRF Director.

Cytometry Specialist II duties include all items outlined above plus:  Independent operation of several cell sorters to successfully execute a wide variety of FACS sorting experiments in a BSL2+ level setting; knowledge of and adherence to standard cell sorting biosafety practices; interfacing with vendor service engineers; assisting in creating and editing facility standard operating procedures (SOPs) and training materials.

 

 

Qualifications

ESSENTIAL QUALIFICATIONS

  • Bachelor's degree in biology, biochemistry, cell biology, genetics, biotechnology or related field is required. 
  • Strong interpersonal and communication skills, both written and verbal. This position requires constant interaction with a large user base of varying personalities and experience, so patience, willingness to teach and troubleshoot, and the ability to interact in a professional and effective manner is critical for success.
  • Independent critical thinking and problem solving ability. Successful instrument troubleshooting, which is a major component of this position, requires implementing a logical deductive workflow, based on knowledge of flow cytometry principles and instrument components, to discover root causes and resolve issues.
  • Flexibility and excellent attention to detail.
  • Aptitude for continued learning and development, especially with regard to complex instrumentation and technology.
  • Experience in a fast-paced, multi-tasking environment and the ability to work independently is required. 
  • Advanced knowledge of Microsoft suite (Word, Excel, PowerPoint) software is essential. 
  • For Cytometry Specialist II level: a minimum of 3-5 years of hands-on analytical flow cytometry and/or cell sorting experience; knowledge of standard cell sorting biosafety practices; and demonstrated knowledge of FACSDiVa and FCS Express or FlowJo software platforms.

 

PREFERRED QUALIFICATIONS

 

Cytometry Specialist I:  Previous flow cytometry knowledge and hands-on experience with routine and advanced multi-color experiments and cell sorting is desirable. One to three years’ work experience in a laboratory environment is preferred.   

 

Cytometry Specialist II:  Hands-on experience and proficiency with the operation and maintenance of current instrumentation in the FCRF is preferred.  Demonstrated experience with advanced flow cytometric data analysis and interpretation is desirable.  Previous experience in a shared resource laboratory environment and in the training of new users is preferred.

 

Schedule:  Monday-Friday, 9:30am-5:30pm. 

Please note that this position may require some flexibility of working hours, including occasional overtime to support research needs.  No weekend hours are required.

 

The final candidate will be required to complete a background check successfully. Submission of a resume and cover letter is required.

 

Princeton University is an Equal Opportunity/Affirmative Action Employer and all qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, sexual orientation, gender identity or expression, national origin, disability status, protected veteran status, or any other characteristic protected by law. EEO IS THE LAW

Standard Weekly Hours

36.25

Eligible for Overtime

Yes

Benefits Eligible

Yes

Probationary Period

90 days

Essential Services Personnel (see policy for detail)

No

Physical Capacity Exam Required

No

Valid Driver's License Required

No

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